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mtt solution  (Biotium)


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    Structured Review

    Biotium mtt solution
    Effects of HNF 14 on cellular metabolic activity in <t>AMD</t> <t>cybrids.</t> Cellular metabolic activity was assessed using an <t>MTT</t> assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
    Mtt Solution, supplied by Biotium, used in various techniques. Bioz Stars score: 95/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtt solution/product/Biotium
    Average 95 stars, based on 172 article reviews
    mtt solution - by Bioz Stars, 2026-04
    95/100 stars

    Images

    1) Product Images from "Characterization of the Effects of a Humanin Fragment Peptide (HNF 14 ) in Age-Related Macular Degeneration"

    Article Title: Characterization of the Effects of a Humanin Fragment Peptide (HNF 14 ) in Age-Related Macular Degeneration

    Journal: Journal of Clinical Medicine

    doi: 10.3390/jcm15051686

    Effects of HNF 14 on cellular metabolic activity in AMD cybrids. Cellular metabolic activity was assessed using an MTT assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
    Figure Legend Snippet: Effects of HNF 14 on cellular metabolic activity in AMD cybrids. Cellular metabolic activity was assessed using an MTT assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

    Techniques Used: Activity Assay, MTT Assay



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    Effects of HNF 14 on cellular metabolic activity in <t>AMD</t> <t>cybrids.</t> Cellular metabolic activity was assessed using an <t>MTT</t> assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
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    Effects of HNF 14 on cellular metabolic activity in <t>AMD</t> <t>cybrids.</t> Cellular metabolic activity was assessed using an <t>MTT</t> assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.
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    MedChemExpress mtt solution
    A Viability <t>of</t> <t>3T3-L1</t> cells treated with the indicated drugs at various concentrations, as determined by <t>MTT</t> assay. B, C Western blot analysis of METTL3 protein expression in 3T3-L1 cells following treatment with individual drugs. D Global m 6 A levels in mRNA from LA-treated 3T3-L1 cells, measured by m 6 A dot blot using an anti-m 6 A antibody. E Quantitative analysis of global mRNA m 6 A levels in LA-treated cells using a colorimetric ELISA kit.
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    Image Search Results


    Effects of HNF 14 on cellular metabolic activity in AMD cybrids. Cellular metabolic activity was assessed using an MTT assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

    Journal: Journal of Clinical Medicine

    Article Title: Characterization of the Effects of a Humanin Fragment Peptide (HNF 14 ) in Age-Related Macular Degeneration

    doi: 10.3390/jcm15051686

    Figure Lengend Snippet: Effects of HNF 14 on cellular metabolic activity in AMD cybrids. Cellular metabolic activity was assessed using an MTT assay in normal and age-related macular degeneration (AMD) transmitochondrial cybrid cells treated with HNF 14 (3.2 µM) for 48 h. Individual data points represent biological replicates, and horizontal bars indicate the mean ± SD ( n = 5 biological replicates). Data were normalized to untreated normal cybrids. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant.

    Article Snippet: AMD and normal cybrids plated in 96-well tissue culture plates were treated with MTT solution (Catalog number: 30006, Biotium, Fremont, CA, USA) at the 48 h time point.

    Techniques: Activity Assay, MTT Assay

    A Viability of 3T3-L1 cells treated with the indicated drugs at various concentrations, as determined by MTT assay. B, C Western blot analysis of METTL3 protein expression in 3T3-L1 cells following treatment with individual drugs. D Global m 6 A levels in mRNA from LA-treated 3T3-L1 cells, measured by m 6 A dot blot using an anti-m 6 A antibody. E Quantitative analysis of global mRNA m 6 A levels in LA-treated cells using a colorimetric ELISA kit.

    Journal: bioRxiv

    Article Title: Licoisoflavone A improves adipose tissue dysfunction in response to diet induced obesity by promoting METTL3 expression

    doi: 10.64898/2025.12.14.694234

    Figure Lengend Snippet: A Viability of 3T3-L1 cells treated with the indicated drugs at various concentrations, as determined by MTT assay. B, C Western blot analysis of METTL3 protein expression in 3T3-L1 cells following treatment with individual drugs. D Global m 6 A levels in mRNA from LA-treated 3T3-L1 cells, measured by m 6 A dot blot using an anti-m 6 A antibody. E Quantitative analysis of global mRNA m 6 A levels in LA-treated cells using a colorimetric ELISA kit.

    Article Snippet: 3T3-L1 cell were incubated with 5 mg/mL MTT solution (MedchemExpress, HY-15924) for 4 h. Dimethylsulfoxide (DMSO) was added to each well, and the plate was shaken for 10 min to completely dissolve the crystals.

    Techniques: MTT Assay, Western Blot, Expressing, Dot Blot, Enzyme-linked Immunosorbent Assay